Search results for "MESH: Recombination"

showing 2 items of 2 documents

Toxicity of ligand-dependent Cre recombinases and generation of a conditional Cre deleter mouse allowing mosaic recombination in peripheral tissues.

2007

Ligand-activated Cre recombinases are widely used for studying gene function in vitro and in conditional mouse models. To compare ligand-dependent Cre recombinases, different Cre estrogen receptor fusions were introduced into the ROSA26 locus of embryonic stem (ES) cells and assayed for genotoxicity and recombination efficiency. Of the tested recombinases, the CreERT2 variant showed no toxicity and was highly responsive to ligand induction. To constitutively express CreERT2 in mice and also to clarify whether the CreERT2 system displays background activity, we generated a knock-in mouse line harboring the CreERT2 coding region under the control of the ROSA26 locus. Analysis of this ROSA26-…

MESH: IntegrasesPhysiologyMESH: Mice TransgenicTransgeneMice TransgenicMESH: Flow Cytometry[SDV.CAN]Life Sciences [q-bio]/CancerBiologyLigandsGreen fluorescent proteinMiceMESH: Brain[SDV.CAN] Life Sciences [q-bio]/CancerGenes ReporterGene expressionGeneticsRecombinaseMESH: LigandsAnimalsMESH: AnimalsMESH: Models GeneticGeneMESH: MiceRecombination GeneticIntegrasesModels GeneticMosaicismMESH: GenomicsMESH: Genes ReporterMESH: DNABrainDNAGenomicsFlow CytometryEmbryonic stem cellMolecular biologyPhenotypeDisease Models AnimalMESH: Gene DeletionMESH: Recombination GeneticMESH: MosaicismMESH: Disease Models AnimalFunctional genomicsGene Deletion
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Evolutionary Dissection of the Dot/Icm System Based on Comparative Genomics of 58 Legionella Species

2019

14 páginas, 2 figuras, 2 tablas

MESH: Selection GeneticLegionella pneumophilaMESH: Bacterial Proteins/metabolism*Negative selectionPositive-selectionDot/Icm systemMESH: PhylogenyNegative-selectionPhylogenyMESH: Evolution MolecularRecombination Genetic0303 health sciencesEffectorMESH: GenomicsGenomics3. Good healthCell biologypositive-selectionDiversifying-selectionMESH: Recombination GeneticMESH: Membrane ProteinsResearch ArticleSignal peptidenegative-selectionEvolutionLegionellaMESH: Carrier ProteinsBiologyMESH: Bacterial Proteins/geneticsEvolution MolecularType IV Secretion Systems03 medical and health sciencesdiversifying-selectionMESH: Type IV Secretion Systems*Bacterial Proteins[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN]evolutionGeneticsSecretionSelection GeneticEcology Evolution Behavior and SystematicsMESH: Legionella/classification030304 developmental biologyComparative genomicsMESH: Legionella/metabolism030306 microbiologyMESH: Legionella/geneticsMembrane ProteinsPeriplasmic spacebiology.organism_classificationCytoplasmCarrier Proteins
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